Nurse-Managed Health Clinic Investment Act of 2009 - Amends the Public Health Service Act to direct the Secretary of Health and Human Services (HHS) to award grants for operating nurse-managed health clinics. Defines "nurse-managed health clinic" as a nurse-practice arrangement that provides primary care or wellness services to underserved or vulnerable populations regardless of insurance status or ability to pay and that is associated with an educational institution, health center, or social services agency. Requires the Secretary to provide technical assistance and services to such clinics in meeting the requirements of this Act and to develop and implement a plan for evaluating such clinics.