Biofuels Investment Trust Fund Act - Establishes a Biofuels Investment Trust Fund consisting of transferred amounts received in the U.S. Treasury from duties collected on imports of ethyl alcohol or any mixture containing ethyl alcohol that is to be used as a fuel or in producing a mixture of gasoline and alcohol, a mixture of a special fuel and alcohol, or any other mixture to be used as a fuel (including motor fuel). Directs the Secretary of Energy to use amounts in the Trust Fund to: (1) provide assistance to farmers, producers, biorefiners, researchers, universities, and other persons or entities involved in the research, development, and deployment, or production of biofuels, especially for cellulosic ethanol production; or (2) advance research, development, and deployment of biofuels, especially cellulosic ethanol produced from biomass feedstocks.